Sequencing:Article Title: Draft genome sequences of Lactiplantibacillus plantarum strains KABP062 and KABP063 isolated from human feces.
Article Snippet: .. Total reads 17,856,196 20,853,774 Number of contigs 53 38 Genome size (bp) 3,262,171 3,251,349 N50 (bp) 130,217 247,484 Depth 175× 190× G + C content (%) 44.5 44.4 BUSCO completeness 98.39% complete (122 C, 2 D, 0 F, 0 M; 124 total) 98.39% complete (122 C, 2 D, 0 F, 0 M; 124 total) Total genes (Prokaryotic Genome Annotation Pipeline) 3,152 3,149 Protein-coding sequences 3,020 3,011 tRNA genes 66 66 5S rRNA 5 6 16S rRNA 3 1 23S rRNA 1 1 16S rRNA sequencing best hit (%) 99.93 (Lp JCM 1149) 99.93 (Lp JCM 1149) Average nucleotide identity of L. plantarum ATCC 14917 (%) 98.83 98.83 aLp, Lactiplantibacillus plantarum. ..
Article Title: Endocrine-targeting therapies shift the breast microbiome to reduce estrogen receptor-α breast cancer risk.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies F4/80 (D2S9R) XP Rabbit mAb Cell Signaling Cat #: 70076 RRID:AB_2799771 GLUT1 Recombinant Rabbit Monoclonal Antibody Invitrogen Cat #: SA0377; RRID:AB_2941981 Lipoteichoic Acid Monoclonal Antibody Invitrogen Cat #: MA1-40134 RRID:AB_1076514 Lipopolysaccharide Polyclonal Antibody LSBio Cat #: LS-C375096 Ki-67 (D3B5) Rabbit mAb Cell Signaling Cat #: 12202 RRID:AB_2620142 Hexokinase I (C35C4) Rabbit mAb Cell Signaling Cat #: 2024 RRID:AB_2116996 PKM1/2 (C103A3) Rabbit mAb Cell Signaling Cat #: 3190 RRID:AB_2163695 alpha-Tubulin Antibody Cell Signaling Cat #: 2144 RRID:AB_2210548 Bacterial and virus strains Lactobacillus paracasei NI327 Over-the-counter probiotic N/A Bifidobacterium breve NI312 Over-the-counter probiotic N/A Bifidobacterium lactis NI349 Over-the-counter probiotic N/A Lactobacillus acidophilus NI317 Over-the-counter probiotic N/A Lactobacillus plantarum NI329 Over-the-counter probiotic N/A Lactobacillus rhamnoses NI332 Over-the-counter probiotic N/A Bifidobacterium bifidum Over-the-counter probiotic N/A Biological samples Postmenopausal ER + tumors Wake Forest University Comprehensive Cancer Center Table S2 IRB00026366 Chemicals, peptides, and recombinant proteins (Z)-4-Hydroxytamoxifen Tocris Cat #: 0999 Critical commercial assays DNeasy PowerLyzer PowerSoil Kit Qiagen Cat #: 12855-100 16S Sequencing CosmosID N/A Untargeted Metabolomics Metabolon N/A Untargeted Metabolomics Proteomics and Metabolomics Shared Resource of the Wake Forest School of Medicine and Wake Forest Baptist Comprehensive Cancer Center (NIH/NCI P30 CA12197) N/A Deposited data Raw data of 16S rRNA sequencing This paper NCBI Sequence Read Archive: SUB14616768 Raw data of untargeted metabolomics This paper Figshare: https://doi.org/10.6084/m9. figshare.27292473.v1 Experimental models: Cell lines MCF-7 ATCC Cat #: HTB-22 RRID:CVCL_0031 ZR-75-1 ATCC Cat #:CRL-1500 RRID:CVCL_0588 (Continued on next page) e1 Cell Reports Medicine 6, 101880, January 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER HMT-3522 S1 Dr. Pierre Vidi (Institut de Cancerologie de l’Ouest, Angers, France) Holmes et al.85 4T1.2ER+ Bethany Kerr and Katherine L. Cook Lab Langsten et al., 202386 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratories RRID:IMSR_JAX:000664 Mouse: BALB/cJ Jackson Laboratories RRID:IMSR_JAX:000651 Mouse: B6.FVB-Tg(MMTV-PyVT) 634Mul/LellJ Jackson Laboratories RRID:IMSR_JAX:022974 Non-human primate: Cynomolgus Macaques (Macaca fascicularis) Institut Pertanian Bogor or Charles River Primates Arnone et al.61 Cline et al.87 Software and algorithms inForm Tissue Analysis Software Akoya BioSciences RRID:SCR_019155 VIS Image Analysis Software Visiopharm RRID:SCR_021711 Image Lab Software Bio Rad RRID:SCR_014210 GraphPad Prism GraphPad RRID:SCR_002798 Seahorse Wave Agilent Technologies RRID:SCR_014526 RTCA Software Agilent Technologies RRID:SCR_014821 MetaboAnalyst McGill University; Montreal; Canada RRID:SCR_015539 Biorender Biorender RRID:SCR_018361 Other Mantra Quantitative Pathology Image System Akoya Biosciences N/A Agilent Seahorse XFe96 Analyzer Agilent Technologies RRID:SCR_019545 Seahorse XF Cell Mito Stress Test Kit Agilent Technologies Cat #:103015-100 Seahorse XF DMEM medium, pH 7.4, 500 mL Agilent Technologies Cat #:103575-100 Seahorse XF 1.0 M glucose solution, 50 mL Agilent Technologies Cat #: 103577-100 Seahorse XF 100 mM pyruvate solution, 50 mL Agilent Technologies Cat #: 103578-100 Seahorse XF 200 mM glutamine solution, 50 mL Agilent Technologies Cat #: 103579-100 Seahorse XF Calibrant Solution 500 mL Agilent Technologies Cat #: 100840-000 Seahorse FluxPaks Agilent Technologies Cat #: 103792-100 Agilent xCELLigence RTCA DP - Cell Invasion & Migration Agilent Technologies RRID:SCR_019037 Agilent xCELLigence RTCA DP E-Plate 16; 36 Plates Agilent Technologies Cat #: 5469813001 Life Technologies QuantStudio 3 Real-Time PCR System ThermoFisher Scientific RRID:SCR_020238 BioRad Trans Blot Turbo system Biorad RRID:SCR_023156 Bio Rad ChemiDoc MP Imaging System Biorad RRID:SCR_019037 DMEM, high glucose, pyruvate Gibco Cat #: 11995065 RPMI 1640 Medium Gibco Cat #: 11875093 DMEM/F-12, HEPES Gibco Cat #: 11330057 Ovine Prolactin NIDDK Cat #: NIDDK-oPRL-21 Insulin solution human Sigma Cat #: I9278 Hydrocortisone Sigma Cat #: H0888 b-Estradiol Sigma Cat #: E2758 (Continued on next page) Cell Reports Medicine 6, 101880, January 21, 2025 e2
Article Title: Fermented Fish Products: A Comprehensive Overview of Traditional Processing Techniques, Varieties, and Their Health Benefits
Article Snippet: In vitro study , — , Gram staining, catalase test, PCR amplification, BLAST analysis, UV–Vis spectrophotometer, pH meter, centrifuge , ↑ Survival at low pH (2–3) ↑ bile tolerance (0.3%–1%) ↑ Cell surface hydrophobicity (27%–41%) ↑ auto‐aggregation ↓ Hemolytic activity ↑ Antimicrobial activity ↑ DPPH radical scavenging activity ↓ Antibiotic resistance , The LAB isolated from shidal exhibited potential probiotic attributes, including survival under gastrointestinal‐like conditions, antimicrobial activity, and aggregation. L. plantarum and P . pentosaceus were identified as the most promising for use as probiotic cultures in functional fermented fish products , Gupta et al. ( ) . .. In vitro antimicrobial assay , — , Gel electrophoresis and BLAST analysis, catalase, PCR amplification, 16S rRNA sequencing, well diffusion assay, bacteriocin neutralization assay, light microscopy , ↑ Antimicrobial activity against E. coli O157:H7 (21.26 mm) ↑ S. aureus ATCC 25923 (18.23 mm) ↓ Listeria monocytogenes (5.10 mm) ↑ Crude bacteriocin activity ↓ No activity against L. monocytogenes ↓ pH (acidic medium) , Pediococcus acidilactici PB22 isolated from bekasam exhibited strong antimicrobial activity, primarily due to bacteriocin and organic acid production. , Purwati et al. ( ) . .. In vitro antagonistic assay , 20 μL LAB culture and 50 μL neutralized cell‐free supernatant , Incubator (37°C), centrifuge, pH meter, spectrophotometer, thermal cycler, and electrophoresis unit , ↑ Isolates showed inhibition against S. aureus ATCC 6538 and P. aeruginosa ATCC 27853 ↑ Strongest inhibition by the isolate Aerococcus NJ‐20 ↓ No inhibition against P. aeruginosa ↓ No bacteriocin activity ↑ NJ‐20 tolerant to 6.5% NaCl and pH 9.6 ↓ Growth at pH 4.4 or 18% NaCl , LAB isolates from peda exhibited varying levels of antagonistic activity, with Aerococcus NJ‐20 showing the strongest inhibition against S. aureus . , Putra et al. ( ) .
Article Title: Addressing controversy in Fusobacterium nomenclature: what exactly does " F. nucleatum " refer to?
Article Snippet: .. For example, while F. nucleatum sensu lato are enriched in colorectal cancer (CRC),24,25 recent studies have shown that it is specifically F. nucleatum subsp. animalis that is significantly associated with CRC.13,16,28,31–33 Recent work from our group aligned with these observations, where we showed that even though isolates identified as F. nucleatum subsp. animalis by 16S rRNA sequencing formed two distinct lineages, only one of these lineages, “Fna C2” (represented by the F. nucleatum subsp. animalis type strain ATCC 51191), was enriched in CRC.16 As this work increased resolution of the F. nucleatum sensu lato lineages to highlight the importance of F. nucleatum subspecies animalis in CRC, we elected to maintain the sensu lato nomenclature in that publication,16 instead of updating to species-level names, for two important reasons. ..
Article Title: Addressing controversy in Fusobacterium nomenclature: what exactly does “ F. nucleatum ” refer to?
Article Snippet: .. For example, while F. nucleatum sensu lato are enriched in colorectal cancer (CRC), , recent studies have shown that it is specifically F. nucleatum subsp. animalis that is significantly associated with CRC., , , Recent work from our group aligned with these observations, where we showed that even though isolates identified as F. nucleatum subsp. animalis by 16S rRNA sequencing formed two distinct lineages, only one of these lineages, “ Fna C2” (represented by the F. nucleatum subsp. animalis type strain ATCC 51191), was enriched in CRC. ..
Article Title: Gut Pathogens and Cytokine Profiles in Autism: A Multi-Biosample Analysis.
Article Snippet: To explore immune, microbial, and gene expression alterations in children with autism spectrum disorder (ASD), assessing their potential as diagnostic markers.. Thirty participants, 20 with ASD (15 male, 5 female) and 10 healthy controls, underwent collection of saliva, blood, and stool.. Serum cytokines (IL1β, IL6, IL17A, TNFα) were quantified via ELISA.
Recombinant:Article Title: Endocrine-targeting therapies shift the breast microbiome to reduce estrogen receptor-α breast cancer risk.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies F4/80 (D2S9R) XP Rabbit mAb Cell Signaling Cat #: 70076 RRID:AB_2799771 GLUT1 Recombinant Rabbit Monoclonal Antibody Invitrogen Cat #: SA0377; RRID:AB_2941981 Lipoteichoic Acid Monoclonal Antibody Invitrogen Cat #: MA1-40134 RRID:AB_1076514 Lipopolysaccharide Polyclonal Antibody LSBio Cat #: LS-C375096 Ki-67 (D3B5) Rabbit mAb Cell Signaling Cat #: 12202 RRID:AB_2620142 Hexokinase I (C35C4) Rabbit mAb Cell Signaling Cat #: 2024 RRID:AB_2116996 PKM1/2 (C103A3) Rabbit mAb Cell Signaling Cat #: 3190 RRID:AB_2163695 alpha-Tubulin Antibody Cell Signaling Cat #: 2144 RRID:AB_2210548 Bacterial and virus strains Lactobacillus paracasei NI327 Over-the-counter probiotic N/A Bifidobacterium breve NI312 Over-the-counter probiotic N/A Bifidobacterium lactis NI349 Over-the-counter probiotic N/A Lactobacillus acidophilus NI317 Over-the-counter probiotic N/A Lactobacillus plantarum NI329 Over-the-counter probiotic N/A Lactobacillus rhamnoses NI332 Over-the-counter probiotic N/A Bifidobacterium bifidum Over-the-counter probiotic N/A Biological samples Postmenopausal ER + tumors Wake Forest University Comprehensive Cancer Center Table S2 IRB00026366 Chemicals, peptides, and recombinant proteins (Z)-4-Hydroxytamoxifen Tocris Cat #: 0999 Critical commercial assays DNeasy PowerLyzer PowerSoil Kit Qiagen Cat #: 12855-100 16S Sequencing CosmosID N/A Untargeted Metabolomics Metabolon N/A Untargeted Metabolomics Proteomics and Metabolomics Shared Resource of the Wake Forest School of Medicine and Wake Forest Baptist Comprehensive Cancer Center (NIH/NCI P30 CA12197) N/A Deposited data Raw data of 16S rRNA sequencing This paper NCBI Sequence Read Archive: SUB14616768 Raw data of untargeted metabolomics This paper Figshare: https://doi.org/10.6084/m9. figshare.27292473.v1 Experimental models: Cell lines MCF-7 ATCC Cat #: HTB-22 RRID:CVCL_0031 ZR-75-1 ATCC Cat #:CRL-1500 RRID:CVCL_0588 (Continued on next page) e1 Cell Reports Medicine 6, 101880, January 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER HMT-3522 S1 Dr. Pierre Vidi (Institut de Cancerologie de l’Ouest, Angers, France) Holmes et al.85 4T1.2ER+ Bethany Kerr and Katherine L. Cook Lab Langsten et al., 202386 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratories RRID:IMSR_JAX:000664 Mouse: BALB/cJ Jackson Laboratories RRID:IMSR_JAX:000651 Mouse: B6.FVB-Tg(MMTV-PyVT) 634Mul/LellJ Jackson Laboratories RRID:IMSR_JAX:022974 Non-human primate: Cynomolgus Macaques (Macaca fascicularis) Institut Pertanian Bogor or Charles River Primates Arnone et al.61 Cline et al.87 Software and algorithms inForm Tissue Analysis Software Akoya BioSciences RRID:SCR_019155 VIS Image Analysis Software Visiopharm RRID:SCR_021711 Image Lab Software Bio Rad RRID:SCR_014210 GraphPad Prism GraphPad RRID:SCR_002798 Seahorse Wave Agilent Technologies RRID:SCR_014526 RTCA Software Agilent Technologies RRID:SCR_014821 MetaboAnalyst McGill University; Montreal; Canada RRID:SCR_015539 Biorender Biorender RRID:SCR_018361 Other Mantra Quantitative Pathology Image System Akoya Biosciences N/A Agilent Seahorse XFe96 Analyzer Agilent Technologies RRID:SCR_019545 Seahorse XF Cell Mito Stress Test Kit Agilent Technologies Cat #:103015-100 Seahorse XF DMEM medium, pH 7.4, 500 mL Agilent Technologies Cat #:103575-100 Seahorse XF 1.0 M glucose solution, 50 mL Agilent Technologies Cat #: 103577-100 Seahorse XF 100 mM pyruvate solution, 50 mL Agilent Technologies Cat #: 103578-100 Seahorse XF 200 mM glutamine solution, 50 mL Agilent Technologies Cat #: 103579-100 Seahorse XF Calibrant Solution 500 mL Agilent Technologies Cat #: 100840-000 Seahorse FluxPaks Agilent Technologies Cat #: 103792-100 Agilent xCELLigence RTCA DP - Cell Invasion & Migration Agilent Technologies RRID:SCR_019037 Agilent xCELLigence RTCA DP E-Plate 16; 36 Plates Agilent Technologies Cat #: 5469813001 Life Technologies QuantStudio 3 Real-Time PCR System ThermoFisher Scientific RRID:SCR_020238 BioRad Trans Blot Turbo system Biorad RRID:SCR_023156 Bio Rad ChemiDoc MP Imaging System Biorad RRID:SCR_019037 DMEM, high glucose, pyruvate Gibco Cat #: 11995065 RPMI 1640 Medium Gibco Cat #: 11875093 DMEM/F-12, HEPES Gibco Cat #: 11330057 Ovine Prolactin NIDDK Cat #: NIDDK-oPRL-21 Insulin solution human Sigma Cat #: I9278 Hydrocortisone Sigma Cat #: H0888 b-Estradiol Sigma Cat #: E2758 (Continued on next page) Cell Reports Medicine 6, 101880, January 21, 2025 e2
Virus:Article Title: Endocrine-targeting therapies shift the breast microbiome to reduce estrogen receptor-α breast cancer risk.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies F4/80 (D2S9R) XP Rabbit mAb Cell Signaling Cat #: 70076 RRID:AB_2799771 GLUT1 Recombinant Rabbit Monoclonal Antibody Invitrogen Cat #: SA0377; RRID:AB_2941981 Lipoteichoic Acid Monoclonal Antibody Invitrogen Cat #: MA1-40134 RRID:AB_1076514 Lipopolysaccharide Polyclonal Antibody LSBio Cat #: LS-C375096 Ki-67 (D3B5) Rabbit mAb Cell Signaling Cat #: 12202 RRID:AB_2620142 Hexokinase I (C35C4) Rabbit mAb Cell Signaling Cat #: 2024 RRID:AB_2116996 PKM1/2 (C103A3) Rabbit mAb Cell Signaling Cat #: 3190 RRID:AB_2163695 alpha-Tubulin Antibody Cell Signaling Cat #: 2144 RRID:AB_2210548 Bacterial and virus strains Lactobacillus paracasei NI327 Over-the-counter probiotic N/A Bifidobacterium breve NI312 Over-the-counter probiotic N/A Bifidobacterium lactis NI349 Over-the-counter probiotic N/A Lactobacillus acidophilus NI317 Over-the-counter probiotic N/A Lactobacillus plantarum NI329 Over-the-counter probiotic N/A Lactobacillus rhamnoses NI332 Over-the-counter probiotic N/A Bifidobacterium bifidum Over-the-counter probiotic N/A Biological samples Postmenopausal ER + tumors Wake Forest University Comprehensive Cancer Center Table S2 IRB00026366 Chemicals, peptides, and recombinant proteins (Z)-4-Hydroxytamoxifen Tocris Cat #: 0999 Critical commercial assays DNeasy PowerLyzer PowerSoil Kit Qiagen Cat #: 12855-100 16S Sequencing CosmosID N/A Untargeted Metabolomics Metabolon N/A Untargeted Metabolomics Proteomics and Metabolomics Shared Resource of the Wake Forest School of Medicine and Wake Forest Baptist Comprehensive Cancer Center (NIH/NCI P30 CA12197) N/A Deposited data Raw data of 16S rRNA sequencing This paper NCBI Sequence Read Archive: SUB14616768 Raw data of untargeted metabolomics This paper Figshare: https://doi.org/10.6084/m9. figshare.27292473.v1 Experimental models: Cell lines MCF-7 ATCC Cat #: HTB-22 RRID:CVCL_0031 ZR-75-1 ATCC Cat #:CRL-1500 RRID:CVCL_0588 (Continued on next page) e1 Cell Reports Medicine 6, 101880, January 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER HMT-3522 S1 Dr. Pierre Vidi (Institut de Cancerologie de l’Ouest, Angers, France) Holmes et al.85 4T1.2ER+ Bethany Kerr and Katherine L. Cook Lab Langsten et al., 202386 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratories RRID:IMSR_JAX:000664 Mouse: BALB/cJ Jackson Laboratories RRID:IMSR_JAX:000651 Mouse: B6.FVB-Tg(MMTV-PyVT) 634Mul/LellJ Jackson Laboratories RRID:IMSR_JAX:022974 Non-human primate: Cynomolgus Macaques (Macaca fascicularis) Institut Pertanian Bogor or Charles River Primates Arnone et al.61 Cline et al.87 Software and algorithms inForm Tissue Analysis Software Akoya BioSciences RRID:SCR_019155 VIS Image Analysis Software Visiopharm RRID:SCR_021711 Image Lab Software Bio Rad RRID:SCR_014210 GraphPad Prism GraphPad RRID:SCR_002798 Seahorse Wave Agilent Technologies RRID:SCR_014526 RTCA Software Agilent Technologies RRID:SCR_014821 MetaboAnalyst McGill University; Montreal; Canada RRID:SCR_015539 Biorender Biorender RRID:SCR_018361 Other Mantra Quantitative Pathology Image System Akoya Biosciences N/A Agilent Seahorse XFe96 Analyzer Agilent Technologies RRID:SCR_019545 Seahorse XF Cell Mito Stress Test Kit Agilent Technologies Cat #:103015-100 Seahorse XF DMEM medium, pH 7.4, 500 mL Agilent Technologies Cat #:103575-100 Seahorse XF 1.0 M glucose solution, 50 mL Agilent Technologies Cat #: 103577-100 Seahorse XF 100 mM pyruvate solution, 50 mL Agilent Technologies Cat #: 103578-100 Seahorse XF 200 mM glutamine solution, 50 mL Agilent Technologies Cat #: 103579-100 Seahorse XF Calibrant Solution 500 mL Agilent Technologies Cat #: 100840-000 Seahorse FluxPaks Agilent Technologies Cat #: 103792-100 Agilent xCELLigence RTCA DP - Cell Invasion & Migration Agilent Technologies RRID:SCR_019037 Agilent xCELLigence RTCA DP E-Plate 16; 36 Plates Agilent Technologies Cat #: 5469813001 Life Technologies QuantStudio 3 Real-Time PCR System ThermoFisher Scientific RRID:SCR_020238 BioRad Trans Blot Turbo system Biorad RRID:SCR_023156 Bio Rad ChemiDoc MP Imaging System Biorad RRID:SCR_019037 DMEM, high glucose, pyruvate Gibco Cat #: 11995065 RPMI 1640 Medium Gibco Cat #: 11875093 DMEM/F-12, HEPES Gibco Cat #: 11330057 Ovine Prolactin NIDDK Cat #: NIDDK-oPRL-21 Insulin solution human Sigma Cat #: I9278 Hydrocortisone Sigma Cat #: H0888 b-Estradiol Sigma Cat #: E2758 (Continued on next page) Cell Reports Medicine 6, 101880, January 21, 2025 e2
In Vitro:Article Title: Fermented Fish Products: A Comprehensive Overview of Traditional Processing Techniques, Varieties, and Their Health Benefits
Article Snippet: In vitro study , — , Gram staining, catalase test, PCR amplification, BLAST analysis, UV–Vis spectrophotometer, pH meter, centrifuge , ↑ Survival at low pH (2–3) ↑ bile tolerance (0.3%–1%) ↑ Cell surface hydrophobicity (27%–41%) ↑ auto‐aggregation ↓ Hemolytic activity ↑ Antimicrobial activity ↑ DPPH radical scavenging activity ↓ Antibiotic resistance , The LAB isolated from shidal exhibited potential probiotic attributes, including survival under gastrointestinal‐like conditions, antimicrobial activity, and aggregation. L. plantarum and P . pentosaceus were identified as the most promising for use as probiotic cultures in functional fermented fish products , Gupta et al. ( ) . .. In vitro antimicrobial assay , — , Gel electrophoresis and BLAST analysis, catalase, PCR amplification, 16S rRNA sequencing, well diffusion assay, bacteriocin neutralization assay, light microscopy , ↑ Antimicrobial activity against E. coli O157:H7 (21.26 mm) ↑ S. aureus ATCC 25923 (18.23 mm) ↓ Listeria monocytogenes (5.10 mm) ↑ Crude bacteriocin activity ↓ No activity against L. monocytogenes ↓ pH (acidic medium) , Pediococcus acidilactici PB22 isolated from bekasam exhibited strong antimicrobial activity, primarily due to bacteriocin and organic acid production. , Purwati et al. ( ) . .. In vitro antagonistic assay , 20 μL LAB culture and 50 μL neutralized cell‐free supernatant , Incubator (37°C), centrifuge, pH meter, spectrophotometer, thermal cycler, and electrophoresis unit , ↑ Isolates showed inhibition against S. aureus ATCC 6538 and P. aeruginosa ATCC 27853 ↑ Strongest inhibition by the isolate Aerococcus NJ‐20 ↓ No inhibition against P. aeruginosa ↓ No bacteriocin activity ↑ NJ‐20 tolerant to 6.5% NaCl and pH 9.6 ↓ Growth at pH 4.4 or 18% NaCl , LAB isolates from peda exhibited varying levels of antagonistic activity, with Aerococcus NJ‐20 showing the strongest inhibition against S. aureus . , Putra et al. ( ) .
Nucleic Acid Electrophoresis:Article Title: Fermented Fish Products: A Comprehensive Overview of Traditional Processing Techniques, Varieties, and Their Health Benefits
Article Snippet: In vitro study , — , Gram staining, catalase test, PCR amplification, BLAST analysis, UV–Vis spectrophotometer, pH meter, centrifuge , ↑ Survival at low pH (2–3) ↑ bile tolerance (0.3%–1%) ↑ Cell surface hydrophobicity (27%–41%) ↑ auto‐aggregation ↓ Hemolytic activity ↑ Antimicrobial activity ↑ DPPH radical scavenging activity ↓ Antibiotic resistance , The LAB isolated from shidal exhibited potential probiotic attributes, including survival under gastrointestinal‐like conditions, antimicrobial activity, and aggregation. L. plantarum and P . pentosaceus were identified as the most promising for use as probiotic cultures in functional fermented fish products , Gupta et al. ( ) . .. In vitro antimicrobial assay , — , Gel electrophoresis and BLAST analysis, catalase, PCR amplification, 16S rRNA sequencing, well diffusion assay, bacteriocin neutralization assay, light microscopy , ↑ Antimicrobial activity against E. coli O157:H7 (21.26 mm) ↑ S. aureus ATCC 25923 (18.23 mm) ↓ Listeria monocytogenes (5.10 mm) ↑ Crude bacteriocin activity ↓ No activity against L. monocytogenes ↓ pH (acidic medium) , Pediococcus acidilactici PB22 isolated from bekasam exhibited strong antimicrobial activity, primarily due to bacteriocin and organic acid production. , Purwati et al. ( ) . .. In vitro antagonistic assay , 20 μL LAB culture and 50 μL neutralized cell‐free supernatant , Incubator (37°C), centrifuge, pH meter, spectrophotometer, thermal cycler, and electrophoresis unit , ↑ Isolates showed inhibition against S. aureus ATCC 6538 and P. aeruginosa ATCC 27853 ↑ Strongest inhibition by the isolate Aerococcus NJ‐20 ↓ No inhibition against P. aeruginosa ↓ No bacteriocin activity ↑ NJ‐20 tolerant to 6.5% NaCl and pH 9.6 ↓ Growth at pH 4.4 or 18% NaCl , LAB isolates from peda exhibited varying levels of antagonistic activity, with Aerococcus NJ‐20 showing the strongest inhibition against S. aureus . , Putra et al. ( ) .
Polymerase Chain Reaction:Article Title: Fermented Fish Products: A Comprehensive Overview of Traditional Processing Techniques, Varieties, and Their Health Benefits
Article Snippet: In vitro study , — , Gram staining, catalase test, PCR amplification, BLAST analysis, UV–Vis spectrophotometer, pH meter, centrifuge , ↑ Survival at low pH (2–3) ↑ bile tolerance (0.3%–1%) ↑ Cell surface hydrophobicity (27%–41%) ↑ auto‐aggregation ↓ Hemolytic activity ↑ Antimicrobial activity ↑ DPPH radical scavenging activity ↓ Antibiotic resistance , The LAB isolated from shidal exhibited potential probiotic attributes, including survival under gastrointestinal‐like conditions, antimicrobial activity, and aggregation. L. plantarum and P . pentosaceus were identified as the most promising for use as probiotic cultures in functional fermented fish products , Gupta et al. ( ) . .. In vitro antimicrobial assay , — , Gel electrophoresis and BLAST analysis, catalase, PCR amplification, 16S rRNA sequencing, well diffusion assay, bacteriocin neutralization assay, light microscopy , ↑ Antimicrobial activity against E. coli O157:H7 (21.26 mm) ↑ S. aureus ATCC 25923 (18.23 mm) ↓ Listeria monocytogenes (5.10 mm) ↑ Crude bacteriocin activity ↓ No activity against L. monocytogenes ↓ pH (acidic medium) , Pediococcus acidilactici PB22 isolated from bekasam exhibited strong antimicrobial activity, primarily due to bacteriocin and organic acid production. , Purwati et al. ( ) . .. In vitro antagonistic assay , 20 μL LAB culture and 50 μL neutralized cell‐free supernatant , Incubator (37°C), centrifuge, pH meter, spectrophotometer, thermal cycler, and electrophoresis unit , ↑ Isolates showed inhibition against S. aureus ATCC 6538 and P. aeruginosa ATCC 27853 ↑ Strongest inhibition by the isolate Aerococcus NJ‐20 ↓ No inhibition against P. aeruginosa ↓ No bacteriocin activity ↑ NJ‐20 tolerant to 6.5% NaCl and pH 9.6 ↓ Growth at pH 4.4 or 18% NaCl , LAB isolates from peda exhibited varying levels of antagonistic activity, with Aerococcus NJ‐20 showing the strongest inhibition against S. aureus . , Putra et al. ( ) .
Amplification:Article Title: Fermented Fish Products: A Comprehensive Overview of Traditional Processing Techniques, Varieties, and Their Health Benefits
Article Snippet: In vitro study , — , Gram staining, catalase test, PCR amplification, BLAST analysis, UV–Vis spectrophotometer, pH meter, centrifuge , ↑ Survival at low pH (2–3) ↑ bile tolerance (0.3%–1%) ↑ Cell surface hydrophobicity (27%–41%) ↑ auto‐aggregation ↓ Hemolytic activity ↑ Antimicrobial activity ↑ DPPH radical scavenging activity ↓ Antibiotic resistance , The LAB isolated from shidal exhibited potential probiotic attributes, including survival under gastrointestinal‐like conditions, antimicrobial activity, and aggregation. L. plantarum and P . pentosaceus were identified as the most promising for use as probiotic cultures in functional fermented fish products , Gupta et al. ( ) . .. In vitro antimicrobial assay , — , Gel electrophoresis and BLAST analysis, catalase, PCR amplification, 16S rRNA sequencing, well diffusion assay, bacteriocin neutralization assay, light microscopy , ↑ Antimicrobial activity against E. coli O157:H7 (21.26 mm) ↑ S. aureus ATCC 25923 (18.23 mm) ↓ Listeria monocytogenes (5.10 mm) ↑ Crude bacteriocin activity ↓ No activity against L. monocytogenes ↓ pH (acidic medium) , Pediococcus acidilactici PB22 isolated from bekasam exhibited strong antimicrobial activity, primarily due to bacteriocin and organic acid production. , Purwati et al. ( ) . .. In vitro antagonistic assay , 20 μL LAB culture and 50 μL neutralized cell‐free supernatant , Incubator (37°C), centrifuge, pH meter, spectrophotometer, thermal cycler, and electrophoresis unit , ↑ Isolates showed inhibition against S. aureus ATCC 6538 and P. aeruginosa ATCC 27853 ↑ Strongest inhibition by the isolate Aerococcus NJ‐20 ↓ No inhibition against P. aeruginosa ↓ No bacteriocin activity ↑ NJ‐20 tolerant to 6.5% NaCl and pH 9.6 ↓ Growth at pH 4.4 or 18% NaCl , LAB isolates from peda exhibited varying levels of antagonistic activity, with Aerococcus NJ‐20 showing the strongest inhibition against S. aureus . , Putra et al. ( ) .
Diffusion-based Assay:Article Title: Fermented Fish Products: A Comprehensive Overview of Traditional Processing Techniques, Varieties, and Their Health Benefits
Article Snippet: In vitro study , — , Gram staining, catalase test, PCR amplification, BLAST analysis, UV–Vis spectrophotometer, pH meter, centrifuge , ↑ Survival at low pH (2–3) ↑ bile tolerance (0.3%–1%) ↑ Cell surface hydrophobicity (27%–41%) ↑ auto‐aggregation ↓ Hemolytic activity ↑ Antimicrobial activity ↑ DPPH radical scavenging activity ↓ Antibiotic resistance , The LAB isolated from shidal exhibited potential probiotic attributes, including survival under gastrointestinal‐like conditions, antimicrobial activity, and aggregation. L. plantarum and P . pentosaceus were identified as the most promising for use as probiotic cultures in functional fermented fish products , Gupta et al. ( ) . .. In vitro antimicrobial assay , — , Gel electrophoresis and BLAST analysis, catalase, PCR amplification, 16S rRNA sequencing, well diffusion assay, bacteriocin neutralization assay, light microscopy , ↑ Antimicrobial activity against E. coli O157:H7 (21.26 mm) ↑ S. aureus ATCC 25923 (18.23 mm) ↓ Listeria monocytogenes (5.10 mm) ↑ Crude bacteriocin activity ↓ No activity against L. monocytogenes ↓ pH (acidic medium) , Pediococcus acidilactici PB22 isolated from bekasam exhibited strong antimicrobial activity, primarily due to bacteriocin and organic acid production. , Purwati et al. ( ) . .. In vitro antagonistic assay , 20 μL LAB culture and 50 μL neutralized cell‐free supernatant , Incubator (37°C), centrifuge, pH meter, spectrophotometer, thermal cycler, and electrophoresis unit , ↑ Isolates showed inhibition against S. aureus ATCC 6538 and P. aeruginosa ATCC 27853 ↑ Strongest inhibition by the isolate Aerococcus NJ‐20 ↓ No inhibition against P. aeruginosa ↓ No bacteriocin activity ↑ NJ‐20 tolerant to 6.5% NaCl and pH 9.6 ↓ Growth at pH 4.4 or 18% NaCl , LAB isolates from peda exhibited varying levels of antagonistic activity, with Aerococcus NJ‐20 showing the strongest inhibition against S. aureus . , Putra et al. ( ) .
Neutralization:Article Title: Fermented Fish Products: A Comprehensive Overview of Traditional Processing Techniques, Varieties, and Their Health Benefits
Article Snippet: In vitro study , — , Gram staining, catalase test, PCR amplification, BLAST analysis, UV–Vis spectrophotometer, pH meter, centrifuge , ↑ Survival at low pH (2–3) ↑ bile tolerance (0.3%–1%) ↑ Cell surface hydrophobicity (27%–41%) ↑ auto‐aggregation ↓ Hemolytic activity ↑ Antimicrobial activity ↑ DPPH radical scavenging activity ↓ Antibiotic resistance , The LAB isolated from shidal exhibited potential probiotic attributes, including survival under gastrointestinal‐like conditions, antimicrobial activity, and aggregation. L. plantarum and P . pentosaceus were identified as the most promising for use as probiotic cultures in functional fermented fish products , Gupta et al. ( ) . .. In vitro antimicrobial assay , — , Gel electrophoresis and BLAST analysis, catalase, PCR amplification, 16S rRNA sequencing, well diffusion assay, bacteriocin neutralization assay, light microscopy , ↑ Antimicrobial activity against E. coli O157:H7 (21.26 mm) ↑ S. aureus ATCC 25923 (18.23 mm) ↓ Listeria monocytogenes (5.10 mm) ↑ Crude bacteriocin activity ↓ No activity against L. monocytogenes ↓ pH (acidic medium) , Pediococcus acidilactici PB22 isolated from bekasam exhibited strong antimicrobial activity, primarily due to bacteriocin and organic acid production. , Purwati et al. ( ) . .. In vitro antagonistic assay , 20 μL LAB culture and 50 μL neutralized cell‐free supernatant , Incubator (37°C), centrifuge, pH meter, spectrophotometer, thermal cycler, and electrophoresis unit , ↑ Isolates showed inhibition against S. aureus ATCC 6538 and P. aeruginosa ATCC 27853 ↑ Strongest inhibition by the isolate Aerococcus NJ‐20 ↓ No inhibition against P. aeruginosa ↓ No bacteriocin activity ↑ NJ‐20 tolerant to 6.5% NaCl and pH 9.6 ↓ Growth at pH 4.4 or 18% NaCl , LAB isolates from peda exhibited varying levels of antagonistic activity, with Aerococcus NJ‐20 showing the strongest inhibition against S. aureus . , Putra et al. ( ) .
Light Microscopy:Article Title: Fermented Fish Products: A Comprehensive Overview of Traditional Processing Techniques, Varieties, and Their Health Benefits
Article Snippet: In vitro study , — , Gram staining, catalase test, PCR amplification, BLAST analysis, UV–Vis spectrophotometer, pH meter, centrifuge , ↑ Survival at low pH (2–3) ↑ bile tolerance (0.3%–1%) ↑ Cell surface hydrophobicity (27%–41%) ↑ auto‐aggregation ↓ Hemolytic activity ↑ Antimicrobial activity ↑ DPPH radical scavenging activity ↓ Antibiotic resistance , The LAB isolated from shidal exhibited potential probiotic attributes, including survival under gastrointestinal‐like conditions, antimicrobial activity, and aggregation. L. plantarum and P . pentosaceus were identified as the most promising for use as probiotic cultures in functional fermented fish products , Gupta et al. ( ) . .. In vitro antimicrobial assay , — , Gel electrophoresis and BLAST analysis, catalase, PCR amplification, 16S rRNA sequencing, well diffusion assay, bacteriocin neutralization assay, light microscopy , ↑ Antimicrobial activity against E. coli O157:H7 (21.26 mm) ↑ S. aureus ATCC 25923 (18.23 mm) ↓ Listeria monocytogenes (5.10 mm) ↑ Crude bacteriocin activity ↓ No activity against L. monocytogenes ↓ pH (acidic medium) , Pediococcus acidilactici PB22 isolated from bekasam exhibited strong antimicrobial activity, primarily due to bacteriocin and organic acid production. , Purwati et al. ( ) . .. In vitro antagonistic assay , 20 μL LAB culture and 50 μL neutralized cell‐free supernatant , Incubator (37°C), centrifuge, pH meter, spectrophotometer, thermal cycler, and electrophoresis unit , ↑ Isolates showed inhibition against S. aureus ATCC 6538 and P. aeruginosa ATCC 27853 ↑ Strongest inhibition by the isolate Aerococcus NJ‐20 ↓ No inhibition against P. aeruginosa ↓ No bacteriocin activity ↑ NJ‐20 tolerant to 6.5% NaCl and pH 9.6 ↓ Growth at pH 4.4 or 18% NaCl , LAB isolates from peda exhibited varying levels of antagonistic activity, with Aerococcus NJ‐20 showing the strongest inhibition against S. aureus . , Putra et al. ( ) .
Activity Assay:Article Title: Fermented Fish Products: A Comprehensive Overview of Traditional Processing Techniques, Varieties, and Their Health Benefits
Article Snippet: In vitro study , — , Gram staining, catalase test, PCR amplification, BLAST analysis, UV–Vis spectrophotometer, pH meter, centrifuge , ↑ Survival at low pH (2–3) ↑ bile tolerance (0.3%–1%) ↑ Cell surface hydrophobicity (27%–41%) ↑ auto‐aggregation ↓ Hemolytic activity ↑ Antimicrobial activity ↑ DPPH radical scavenging activity ↓ Antibiotic resistance , The LAB isolated from shidal exhibited potential probiotic attributes, including survival under gastrointestinal‐like conditions, antimicrobial activity, and aggregation. L. plantarum and P . pentosaceus were identified as the most promising for use as probiotic cultures in functional fermented fish products , Gupta et al. ( ) . .. In vitro antimicrobial assay , — , Gel electrophoresis and BLAST analysis, catalase, PCR amplification, 16S rRNA sequencing, well diffusion assay, bacteriocin neutralization assay, light microscopy , ↑ Antimicrobial activity against E. coli O157:H7 (21.26 mm) ↑ S. aureus ATCC 25923 (18.23 mm) ↓ Listeria monocytogenes (5.10 mm) ↑ Crude bacteriocin activity ↓ No activity against L. monocytogenes ↓ pH (acidic medium) , Pediococcus acidilactici PB22 isolated from bekasam exhibited strong antimicrobial activity, primarily due to bacteriocin and organic acid production. , Purwati et al. ( ) . .. In vitro antagonistic assay , 20 μL LAB culture and 50 μL neutralized cell‐free supernatant , Incubator (37°C), centrifuge, pH meter, spectrophotometer, thermal cycler, and electrophoresis unit , ↑ Isolates showed inhibition against S. aureus ATCC 6538 and P. aeruginosa ATCC 27853 ↑ Strongest inhibition by the isolate Aerococcus NJ‐20 ↓ No inhibition against P. aeruginosa ↓ No bacteriocin activity ↑ NJ‐20 tolerant to 6.5% NaCl and pH 9.6 ↓ Growth at pH 4.4 or 18% NaCl , LAB isolates from peda exhibited varying levels of antagonistic activity, with Aerococcus NJ‐20 showing the strongest inhibition against S. aureus . , Putra et al. ( ) .
Isolation:Article Title: Fermented Fish Products: A Comprehensive Overview of Traditional Processing Techniques, Varieties, and Their Health Benefits
Article Snippet: In vitro study , — , Gram staining, catalase test, PCR amplification, BLAST analysis, UV–Vis spectrophotometer, pH meter, centrifuge , ↑ Survival at low pH (2–3) ↑ bile tolerance (0.3%–1%) ↑ Cell surface hydrophobicity (27%–41%) ↑ auto‐aggregation ↓ Hemolytic activity ↑ Antimicrobial activity ↑ DPPH radical scavenging activity ↓ Antibiotic resistance , The LAB isolated from shidal exhibited potential probiotic attributes, including survival under gastrointestinal‐like conditions, antimicrobial activity, and aggregation. L. plantarum and P . pentosaceus were identified as the most promising for use as probiotic cultures in functional fermented fish products , Gupta et al. ( ) . .. In vitro antimicrobial assay , — , Gel electrophoresis and BLAST analysis, catalase, PCR amplification, 16S rRNA sequencing, well diffusion assay, bacteriocin neutralization assay, light microscopy , ↑ Antimicrobial activity against E. coli O157:H7 (21.26 mm) ↑ S. aureus ATCC 25923 (18.23 mm) ↓ Listeria monocytogenes (5.10 mm) ↑ Crude bacteriocin activity ↓ No activity against L. monocytogenes ↓ pH (acidic medium) , Pediococcus acidilactici PB22 isolated from bekasam exhibited strong antimicrobial activity, primarily due to bacteriocin and organic acid production. , Purwati et al. ( ) . .. In vitro antagonistic assay , 20 μL LAB culture and 50 μL neutralized cell‐free supernatant , Incubator (37°C), centrifuge, pH meter, spectrophotometer, thermal cycler, and electrophoresis unit , ↑ Isolates showed inhibition against S. aureus ATCC 6538 and P. aeruginosa ATCC 27853 ↑ Strongest inhibition by the isolate Aerococcus NJ‐20 ↓ No inhibition against P. aeruginosa ↓ No bacteriocin activity ↑ NJ‐20 tolerant to 6.5% NaCl and pH 9.6 ↓ Growth at pH 4.4 or 18% NaCl , LAB isolates from peda exhibited varying levels of antagonistic activity, with Aerococcus NJ‐20 showing the strongest inhibition against S. aureus . , Putra et al. ( ) .
|
ATCC is a verified supplier 
ATCC manufactures this product 